Genotyping MUltiplexed-Sequencing of CRISPR-Localized Editing
CRISPR-Cas9 gene editing is widely used to create genetic modified cells. The genetic editing results in cellular heterogeneity, allelic diversity, and randomness of de-novo sequences, which constitute a major challenge in genotyping CRISPR-Cas9-edited cells.
We introduce a streamlined and cost-effective protocol along with the computational tool GMUSCLE, to sequence and genotype the products of CRISPR-Cas9 gene editing with great depth, high accuracy, high efficiency, and handy utilization.
GMUSCLE enables the quantitative and qualitative identification of the genotypes of CRISPR-Cas9-edited cells from sequencing data.